Performance Analysis of bio-sorption of Heavy Metal and Biodegradation PAH of Isolates Marine Sponges Symbiont Bacteria

Heavy metal pollutants and polycyclic aromatic hydrocarbon contaminants,
also known as PAHs, need attention from related parties, considering that their use is very wide, as well as their relatively high natural releases. Careless and uncontrolled handling has the potential to cause problems for creatures, especially in marine ecosystems. The aim of the study was to determine the performance of marine sponge micro symbiont isolates in heavy metal bio-sorption and PAH biodegradation. Pure isolates were obtained by the scratch method using NA media. The pure isolates from the colonization were converted into isolate suspensions using physiological 0.9% NaCl solution. Suspension adapted 24 hours. The isolate suspension was interacted with heavy metals Cd2+ and As3+, each with a concentration of 100 mg/L and naphthalene and anthracene with a concentration of 1000 mg/L. Interaction time of 5, 10, 15, and 20 days. Results of the analysis showed the bio-sorption capacity of the bacterial isolate Sp6.B2 to Cd2+ = 83.190 %, while Sp8.B1 = 82.240 %. Bio-sorption performance of Sp6.B2 isolates against As3+ = 99.890 %, while Sp8.B1 = 99.894 %. The biodegradation performances of Sp6.B2 isolates had a higher aggressiveness towards naphthalene and anthracene test contaminants than Sp8.B1 isolates. These results indicate that the bio�sorption performance of Sp6.B2 ˃ Sp8.B1 isolates against Cd2+ contaminants and vice versa Sp6.B2 ˂ Sp8.B1 against As3+ bio-sorption, while the biodegradation performance of Sp6.B2 ˃ Sp8.B1 isolates both against naphthalene and anthracene test contaminants. The development of this research in the future is directed at tracing sponge symbiont bacteria that have two functions at once, namely as a biomaterial for
PAH degrading and heavy metal bio-adsorbent